Epitope Mapping Service

What is Epitope Mapping

Epitope Mapping is the process of identifying epitope, the binding site of an antibody to a target antigen.

Epitope mapping, providing specific amino-acid sequence bound with antibody, is essential element

of developing monoclonal antibody for diagnosis and treatment.

GeneOnBioTech provide world class epitope mapping

based on ‘175,000 Peptide array’

EPITOPE MAPPING WITH 175,000 PEPTIDE ARRAY

The number of peptide in peptide array used in Epitope Mapping

Epitope Mapping service of other company use 5,500~20,000 peptides.

Epitope Mapping service of GeneOnBioTech uses 175,000 peptides for more accurate and broad result.

You can receive higher resolution result

which you cannot get from other Epitope Mapping Service.

LINEAR AND CONFORMATIONAL EPITOPE

There are two type of antibody epitope, Linear epitope and Conformational epitope.
They are named after their characters, continuous epitope and Discontinuous epitope.

Because most of other peptide array realize linear form only,

It was hard to identify antibody binding with Conformational epitope.

Our 175,000 peptide array, realized in 3D structure, can identify antigen with discontinuous epitope.

Outline of research service of Epitope Mapping

○ Identify Sample-Peptide Interaction through peptide array experiment

○ Select Top-ranked Interaction depended on A-score

○ Verify selected 3D structure/conformational form

○ (Optional) If antigen is suggested, perform sequence comparison between the antigen and experimentally confirmed peptides.

WORLD-BEST PEPTIDE ARRAY

Gene On Peptide array : < 175,000 peptides of < 15-amino acids

GeneOnBioTech provides world-class level peptide array research service.

GeneOn Epitope Mapping Research/Analysis Service

You can receive Raw data and Research results report through GeneOnBioTech’s Epitope Mapping Research/Analysis service.

○ Raw data

Raw data of 100,000 Peptide accumulated as a double spots.

○ Data analysis
(Example of Data analysis)
Derivation of results through data analysis(Repeatedly detected peptide sequence identification).

○ Results report

(Example of Results report 1)
Provide detailed information of the result(Peptide sequence showing strong interaction).

(Example of Results report 2)
Location information of the Peptide sequence in the sample protein sequence.

○ Report contents

REFERENCE LIST

  1. Foertsch T C, Davis A T, Popov R, von Bojničić-Kninski C., Held F E, Tsogoeva S B, Loeffler F F, Nesterov-Mueller A (2019) Spatial Modes of Laser-Induced Mass Transfer in Micro-Gaps. Applied Sciences 9 (7), 1303
  2. Palermo A, Thelen R, Weber L K, Foertsch T, Rentschler S., Hackert V., Syurik J., Nesterov-Mueller A (2019) Vertical Scanning Interferometry for Label-Free Detection of Peptide-Antibody Interactions, High-Throughput 8 (2).
  3. Palermo A & Nesterov-Mueller A (2019) Serological Number for Characterization of Circulating Antibodies. Int J Mol Sci 20, doi:10.3390/ijms20030604.
  4. Bojnicic-Kninski C, Bykovskaya V, Maerkle F, Popov R, Palermo A, Mattes D S, Weber L K, Ridder B, Foertsch T C, Loeffler F F, Breitling F, and Nesterov-Mueller A (2016) Selective Functionalization of Microstructured Surfaces by Laser-Assisted Particle Transfer Adv Funct. Materials, DOI: 10.1002/adfm.201603299
  5. Loeffler F F, Foertsch T C, Popov R, Mattes D S, Schlageter M, Sedlmayr M, Ridder B, Dang F-X, von Bojničić-Kninski C, Weber LK, Fischer A,Greifenstein J, Bykovskaya V, Buliev I, Bischoff F R, Hahn L, Meier M A R, Bräse S, Powell A K, BalabanT S, Breitling F, Nesterov-Mueller A (2016) High-flexibility combinatorial peptide synthesis with laser-based transfer of monomers in matrix material. Nature Communications, DOI: 10.1038/NCOMMS11844
  6. Maerkle F, Loeffler F F, Schillo S, Foertsch T, Muenster B, Striffler J, Schirwitz C, Bischoff F R, Breitling F, Nesterov-Mueller A (2014) High-Density Peptide Arrays with Combinatorial Laser Fusing, Advanced Materials, V. 26: 3730–3734
  7. Nesterov-Mueller A, Märkle F, Förtsch T, Schillo S, Hahn L, Bykovskaya V, Sedlmayr M, Soehindrijo M, Münster B, Striffler J, Bischoff F R, Breitling F, Loeffler F F (2014) Particle-based microarrays of oligonucleotides and oligopeptides, New and Old Technologies for Generation of Microarrays, Microarrays, 3, 245-262
  8. Loffler F, Cheng YC, Fortsch T, Dorsam E, Bischoff R, Breitling F and Nesterov-Mueller A (2011) Biofunctional Xerography, InTech , in Biotechnology of Biopolymers ISBN 978-953-307-179-4, p 275-290
  9. Nesterov A, König K, Felgenhauer T, Lindenstruth V, Trunk U, Fernandez S, Hausmann M., Bischoff F R, Stadler V und Breitling F. (2008) Precise selective deposition of microparticles on electrodes of microelectronic chips. Rev. Scientific Instruments, 79, 035106.
  10. Beyer* M, Nesterov-Mueller* A (shared first authors), Block I, König K, Felgenhauer T, Fernandez S, Leibe K, Torralba G, Hausmann M, Trunk U, Lindenstruth V, Bischoff FR, Stadler V und Breitling F. (2007) Combinatorial synthesis of peptide arrays onto a computer chip’s surface. Science 318, 1888.