PHASE 1 >> ANTIGEN AND IMMUNIZATION
We use 5 mouse immunization using 0.5-2mg client provided protein or carrier-peptide with 80% purity protein.
Or help antigen design or synthesis with CDI.
CDI include 21,000 human protein library.
PHASE 2 >> FUSION AND SCREENING
Select 2 most reactive subjects and collect immune cells to fuse with myeloma producing several hybridoma.
Screening supernatant fluid of IgG-secreting clone.
PHASE 3 >> SUB-CLONING AND EXPANSION
High quality clones (5~30) are selected to be subclone.
Enlarge them to 23 well plate each, and enlarge to T-25 flask.
Supernatant fluid is delivered for additional customer evaluation.
PHASE 4 >> PRODUCTION, PURIFICATION, QA
Each clones are bottled up, labeled and frozen. Each samples are kept for Mycoplasma test.
Produce in 3 kinds of clone according to requirement.
Evaluate with CDI HuProt™ Human Proteome Microarray. V4.0 after purifying produced antibody.