Protein Microarray Service

GeneOn Protein Microarray Research/Analysis Service

You can receive Raw data and Research results report through GeneOnBioTech’s
Protein Microarray Research/Analysis Service.

○ Raw data

Full Raw data of over 21,000 proteins accumulated in a dual spots.

○ Data analysis

(Eliminate the part that can specify the result(Protein name, Location on the Protein Microarray))
Derivation of results through data analysis(Selection of proteins with strong interaction).

○ Results report

(Eliminating the part that can identify the result(Protein name, Uniprot number, etc.))
Provides detailed information of the result(A protein with strong interaction)

○ Report contents

Research/Analytic Service

You can receive various research/analytic service for each sample and purpose using protein microarray.

○ Antibody test : Antibody specificity / Cross-reactivity test

○ Serum profiling : Serum/Plasma profiling for Biomarker detection

Protein-Protein Interactions

Small molecule-protein Interactions

Just prepare sample.

Just prepare sample. We will take charge of whole experimental process.

Sample preparation method :

○ Protein/Antibody :

send us purified sample over 100µg.

Concentration doesn’t matter. We prefer PBS buffer condition.

Send to our lab with freezed/refrigerated packaged.
A : B-101, Dunsan-dong 1392, Seo-gu, Daejeon, Korea

○ Other Samples :

Ask academic request to check optimal sample condition.
T : 070-4124-9090
@ : gene@geneon.kr

Research Result

You can receive research result report and Raw data within 4 weeks after sample preprocessing.
Research result report includes Experiment details, Introduction, Protocol, Materials & Methods, Sample optimization, Research result outline, Scanning image, Result analysis, Binding protein information and Ontology analysis.

단백질칩, 단백질 칩, 바이오칩, 바이오 칩, 마이크로어레이, 마이크로 어레이, 프로틴 칩, 프로틴칩, Protein chip, Proteinchip, BioChip, Bio chip, 단백질 마이크로 어레이, DNA 마이크로 어레이, MicroArray, Micro Array, Protein microarray, protein micro array

Reference
Autoantibody Profiling

  • Fan W et al. (2019) Sa1679–Multiple rather than specific Antibodies were identified in Irritable Bowel Syndrome using Huprot™ Microarray Approach Gastroenterology 156 (6), Supplement 1, Page S-364
  • Lastwika KJ et al. (2019) Tumor-derived autoantibodies identify malignant pulmonary nodules. Am J Respir Crit Care Med, 199 (10)
  • Ye S et al. (2019) Plasma proteomic and autoantibody profiles reveal the proteomic characteristics involved in longevity families in Bama, China Clin Proteomics,16:22
  • Gowen MF et al. (2018) Baseline antibody profiles predict toxicity in melanoma patients treated with immune checkpoint inhibitors. J. Transl. Med., 16(1):82
  • Bigley V et al. (2018) Biallelic interferon regulatory factor 8 mutation: A complex immunodeficiency syndrome with dendritic cell deficiency, monocytopenia, and immune dysregulation. J. Allergy Clin Immunol, 141(6):2234-2248
  • Bremer HD et al. (2018) ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease Sci.Rep. 8(1):4852
  • Chung JM et al. (2018) Identification of the thioredoxin-Like 2 Autoantibody as a Specific Biomarker for Triple-Negative Breast Cancer. J Breast Cancer 21:87-90
  • Pan J et al. (2017) Identification of Serological Biomarkers for Early Diagnosis of Lung Cancer Using a Protein Array-Based Approach. Mol Cell Proteomics 16 (12):2069-2078
  • Wang J et al. (2017) PTMA, a new identified autoantigen for oral submucous fibrosis, regulates oral submucous fibroblast proliferation and extracellular matrix. Oncotarget. 8(43): 74806–74819.
  • Gupta S et al. (2017) Serum Profiling for Identification of Autoantibody Signatures in Diseases Using Protein Microarrays. Methods Mol Biol 1619:303-315.
  • Gupta S et al. (2017) Evaluation of autoantibody signatures in meningioma patients using human proteome arrays. Oncotarget 8 (35): 58443-58456
  • Chung et al. (2017) Phenotyping and auto-antibody production by liver-infiltrating B cells in primary sclerosing cholangitis and primary biliary cholangitis J. Autoimmun 77:45-54
  • Ogishi et al. (2016) Delineation of autoantibody repertoire through differential proteogenomics in hepatitis C virus-induced cryoglobulinemia. Sci Rep 6:29532.
  • Yang L et al. (2016) Identification of serum biomarkers for gastric cancer diagnosis using a human proteome microarray. Mol Cell Proteomics 15(2):614-23.
  • Fiorentino DF et al. (2016) PUF60: a prominent new target of the autoimmune response in dermatomyositis and Sjögren’s syndrome. Ann Rheum Dis 75(6): 1145–1151.
  • Hu CJ et al. (2016) Identification of Novel Biomarkers for Behcet Disease Diagnosis Using HuProt Array Approach. Mol Cell Proteomics 16(2):147-156
  • Shi L et al. (2016) Application of high-throughput protein array in clinical screening for tumor markers. Int J Clin Exp Med 9:8529-8535.
  • Syed P et al. (2015) Autoantibody profiling of glioma serum samples to identify biomarkers using human proteome arrays Sci Rep 15(5):13895
  • Hu C et al. (2015) Autoantibody profiling on human proteome microarray for biomarker discovery in cerebrospinal fluid and sera of neuropsychiatric lupus. PLoS One 10(5):e0126643
  • Hu CJ et al. (2012) Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays. Mol Cell Proteomics 11(9):669-80.

Autoantibody Specificity and Crossreactivity

  • Venkataraman A et al. (2018) A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nat Methods , in press
  • Ramos-López et al. (2018) Antibody Specificity Profiling using Protein Microarrays. In: Rockberg J., Nilvebrant J. (eds) Epitope Mapping Protocols, Methods in Molecular Biology, vol 1785. e0181251.
  • Washburn N et al. (2017) High-resolution physicochemical characterization of different intravenous immunoglobulin products. PLoS One 2017 Jul 31
  • Sterner E et al. (2017) Therapeutic Antibodies to Ganglioside GD2 Evolved from Highly Selective Germline Antibodies. Cell Rep 20(7):1681-1691
  • Kim YP et al (2014) Effective Therapeutic Approach for Head and Neck Cancer by an Engineered Minibody Targeting the EGFR Receptor. PLoS One. 1;9(12):e113442.
  • Liu S et al. (2014) Characterization of monoclonal antibody’s binding kinetics using oblique-incidence reflectivity difference approach. MAbs 7:110-119
  • Jeong JS et al. (2012) Rapid identification of monospecific monoclonal antibodies using a human proteome microarray. Mol Cell Proteomics 11(6):O111.016253